In the cyanmethemoglobin method, at what wavelength is the hemoglobin measured spectrophotometrically?

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Multiple Choice

In the cyanmethemoglobin method, at what wavelength is the hemoglobin measured spectrophotometrically?

Measuring hemoglobin with the cyanmethemoglobin method hinges on the absorbance of cyanmethemoglobin. After Hb is converted to cyanmethemoglobin, the strongest absorbance occurs around 540 nm, so the spectrophotometer is set to that wavelength to quantify how much Hb is present. This wavelength gives a reliable, proportional signal to Hb concentration, following Beer-Lambert behavior. The other options don’t align with the absorption peak of cyanmethemoglobin, so they would yield weaker signals or less accurate measurements.

Measuring hemoglobin with the cyanmethemoglobin method hinges on the absorbance of cyanmethemoglobin. After Hb is converted to cyanmethemoglobin, the strongest absorbance occurs around 540 nm, so the spectrophotometer is set to that wavelength to quantify how much Hb is present. This wavelength gives a reliable, proportional signal to Hb concentration, following Beer-Lambert behavior. The other options don’t align with the absorption peak of cyanmethemoglobin, so they would yield weaker signals or less accurate measurements.

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